- Title
- miRNA-34b/c regulates mucus secretion in RSV-infected airway epithelial cells by targeting FGFR1
- Creator
- Li, Wenkai; Du, Xizi; Liu, Huijun; Qin, Xiaoqun; Xiao, Gelei; Liu, Chi; Yang, Yu; Yuan, Lin; Yang, Ming; Qin, Ling; Wang, Leyuan; Zhou, Kai; Xiang, Yang; Qu, Xiangping
- Relation
- Journal of Cellular and Molecular Medicine Vol. 25, Issue 22, p. 10565-10574
- Publisher Link
- http://dx.doi.org/10.1111/jcmm.16988
- Publisher
- Wiley-Blackwell
- Resource Type
- journal article
- Date
- 2021
- Description
- Respiratory syncytial virus (RSV) infection in airway epithelial cells is the main cause of bronchiolitis in children. Excessive mucus secretion is one of the primary symbols in RSV related lower respiratory tract infections (RSV-related LRTI). However, the pathological processes of mucus hypersecretion in RSV-infected airway epithelial cells remains unclear. The current study explores the involvement of miR-34b/miR-34c in mucus hypersecretion in RSV-infected airway epithelial cells by targeting FGFR1. First, miR-34b/miR-34c and FGFR1 mRNA were quantified by qPCR in throat swab samples and cell lines, respectively. Then, the luciferase reporters’ assay was designed to verify the direct binding between FGFR1 and miR-34b/miR-34c. Finally, the involvement of AP-1 signalling was assessed by western blot. This study identified that miR-34b/miR-34c was involved in c-Jun-regulated MUC5AC production by targeting FGFR1 in RSV-infected airway epithelial cells. These results provide some useful insights into the molecular mechanisms of mucus hypersecretion which may also bring new potential strategies to improve mucus hypersecretion in RSV disease.
- Subject
- airway epithelial cells; FGFR1; miRNA; mucus secretion; respiratory syncytial virus; SDG 3; Sustainable Development Goals
- Identifier
- http://hdl.handle.net/1959.13/1472706
- Identifier
- uon:48903
- Identifier
- ISSN:1582-1838
- Rights
- © 2021 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. http://creativecommons.org/licenses/by/4.0/
- Language
- eng
- Full Text
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